Sensing Non-Self DNA

Electrochemical impedance sensing of DNA at PNA self assembled monolayer

Monolayers of cysteine linked peptide nucleic acid (PNA) assembled on gold electrodes were investigated for the sensing of DNA recognition. The monolayer was characterized by cyclic voltammetry (CV). The electron transfer through the monolayers was investigated using electrochemical impedance spectroscopy (EIS) in the presence of target DNA and redox marker ions [Fe(CN)6] 3 /4 as hybridization ...

Compressive Sensing DNA Microarrays

Compressive sensing microarrays (CSMs) are DNA-based sensors that operate using group testing and compressive sensing (CS) principles. In contrast to conventional DNA microarrays, in which each genetic sensor is designed to respond to a single target, in a CSM, each sensor responds to a set of targets. We study the problem of designing CSMs that simultaneously account for both the constraints f...

Self-Sensing Solid-State Actuators

2001 Fred Griffith review lecture. Immigration control of DNA in bacteria: self versus non-self.

Bacteria commonly endow their DNA with an identity mark. When DNA is transferred from one bacterium to another strain of the same species, DNA that lacks the identification mark of the recipient strain is recognized as ‘ foreign’ rather than ‘self ’. Foreign DNA is commonly degraded. The first evidence for this discriminatory process was the demonstration of a barrier, albeit incomplete, to the...

Innate Immune Sensing of DNA

When a pathogen attacks, the immune system rapidly mobilizes host defenses in order to reduce the microbial burden and limit damage to the host [1]. Innate immunity is the first line of defense and relies on germ line–encoded pattern recognition receptors (PRRs) such as the Toll-like receptors (TLRs), which sense microbial products that are not normally found on or in mammalian cells. The consi...